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Type of Document Master's Thesis Author Caire, Yuridia Author's Email Address ycaire@nd.edu URN etd-04222005-100834 Title Investigations of proteins important for antibiotic resistance Degree Master of Science Department Chemistry and Biochemistry Advisory Committee
Advisor Name Title Dr. Bradley Smith Committee Member Dr. Paul Helquist Committee Member Dr. Paul Huber Committee Member Keywords
- OXA-10
- BlaI
Date of Defense 2005-04-15 Availability restricted Abstract The objective of my first project was to investigate the interaction of a class D beta-lactamase with different Beta-lactams to explore if this family of enzymes is diffusion-limited or not. This project required isolation and purification of the OXA-10 beta-lactamase overexpressed in E. coli BL21 and the determination of kcat, Km, and Vmax parameters for different beta-lactam antibiotics at different viscosities of the reaction buffer.
The other project that I worked on required the cloning of blaI gene into an expression vector, overexpression of BlaI in E. coli BL21, purification of this protein. This protein is a gene repressor, believed to be involved in inducible expression of beta-lactamases in S. aureus.
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